Authors from Hamamatsu Photonics K.K. will present two papers at the 4th Single Molecule Localisation Microscopy Symposium, on the 27th – 29th August 2014 in London, England. The meeting is aimed at the growing single molecule superresolution microscopy community and provides a platform to diffuse the latest advances in the development of fluorescent proteins and dyes, labelling approaches, buffer systems, data processing and analysis software and last but not least the biological discoveries made using single molecule microscopy.
Super Resolution Imaging of Protein Dynamics in Living Cell with Localisation Algorithm of High-Density Fluorophores, “Wedged Template Matching” by Shigeo Watanabe et al, in collaboration with Dr. Yasushi Okada (Riken) describes the Wedged Template Matching (WTM) algorithm for localising molecules with overlapping emission point spread functions. Importantly, the computation time to reconstruct a high-density final super resolution image is 20X – 1000X faster than other multi-emitter fitting algorithms, suggesting that live cell super resolution imaging will be practical. Compensating for the Impacts of Camera-induced Noise in Super-Resolution Localisation Microscopy by Keith Bennett et al, in collaboration with Dr. Daniel Sage (EPFL, Switzerland)) and Prof. Zhen-li Huang (Huazhong University of Science and Technology, China) compares reconstructed images built from identical datasets for scientific CMOS and EMCCD cameras using different algorithms (both algebraic and statistical methods) to show the effects of technology-specific camera imperfections on the reconstructed images. Both papers include experimental data.
Hamamatsu Photonics UK will be also be exhibiting at the conference.